After getting the materials, our team started to conduct the experiment by first numbering the tubes one through eight. Then we measured out 10mL of the buffer into the 15mL conical tube, and then poured the buffer solution into the Erlenmeyer flask. Next, our team placed the funnel into the flask and carefully one member poured the lysozyme into the flask. After waiting five minutes for the lysozyme to dissolve, another member swirled the flask to get rid of any clinging powder. Next, we each slowly added the salt while swirling the flask. The solution was cloudy at first, because our team had the highest concentrate.
After looking at the observations, I suspected some type of bias because the numbers rose so steeply from sample five to sample six. And it dropped sharply from sample one sample two. The sizes of the crystals were very close to what I guessed. I got one size from three fields of view and averaged them and rounded them up.
Most of the crystals were transparent and had light gray edges, and had these basic shapes:
The best sample so far is sample eight because it has the most and the largest protein crystals.
After the second day there were more and bigger crystals in the smaller concentrations. The largest crystals were in sample three and two. Samples five and six had the biggest increases in crystal production. But many of the other samples also had large increases.
There wasn't much of an increase in size, except in sample three and two. I used the same steps in averaging as the above examples.
The largest crystals were in sample three. On day two most of the crystals were bulkier and more evenly distributed. They had just about the same color as on the first day, (transparent with light gray edges) but the crystal samples from three to eight were wider. The crystals included these shapes:
If I were a surgeon I would make a difference in the world because I could cure people's wounds, and perform all types of surgeries depending on what type of doctor or surgeon I will be. Because accidents happen everywhere and most of the average Americans live a sedentary lifestyle a surgeon can really change someone by saving or bettering their lives.
This is isn't a very boring career either because it takes very hard work and concentration to go through medical school and learn new techniques and surgeries. It also takes a lot of guts and training to do the actual surgery, especially the first surgery. You also can't fall asleep in an appendectomy or heart surgery. Another tough lesson in becoming a surgeon is defeating the immense amount of pressure but the reward of helping someone is priceless.
Molecular biology can help in this aspect also because I'll get a better understanding in the way cells react to diseases. I could also know how they are made and their structural outline and components. I would need a basic understanding of proteins, cells and bacteria because these things can cause different reactions in our bodies.
There is also a need for surgeons because more and more people die and receive injuries from serious accidents everyday. If there were more doctors and surgeons there could be better treatment and more available doctors for the patients.
There are many important parts about being a surgeon but helping and saving lives is the most important. Even though this career is very challenging it is also very interesting. With the endless amounts of injuries there is a desperate call for more surgeons and with structural biology I can better understand the making of medicines and vital proteins and cells in the human body.